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Sophia Karandashova

Sophia KarandashovaEducation:

Oregon State University, Honors B.S. in Microbiology (2008); Stephen F. Austin State University, M.S. in Biotechnology (2010)
Hometown: Moscow, Russia
PhD Advisor: Judith Voynow, MD
Graduate Program: CCTR (Cancer and Molecular Medicine)

 

Research Interests:

Sophia’s main research interests are microbiology and pulmonary disease. In particular, Sophia is passionate about translational applications of basic research, and enjoys working with animal models. Her master’s thesis focused on enhancing a rabbit model of complex pleural injury by expressing human proteins associated with the fibrinolytic system (in particular, human plasminogen activator inhibitor-1), in an attempt to manipulate fibrin turnover in the pleural space. This model is designed to further the development of novel molecular-targeting therapies for clearing pleural loculations that could be possible alternatives to surgery.

Personal Bio:

Sophia was born in Moscow, Russia and immigrated to the U.S. when she was eight, eventually moving to Richmond, where she lived until leaving for college. After graduating from OSU and earning a master’s at SFASU, she worked as a research associate for the UT Health Science Center in Tyler, Texas in a lab that focuses on pulmonary and pleural diseases. She has a wide variety of interests, from ballroom dancing to skiing. She especially enjoys writing and reading fiction. She’s very happy to be back on the East Coast, and is enjoying rediscovering Richmond.

Honors, Awards, and Grants:

Children’s Hospital Foundation (CHF) Research Grant (2016-2017) “Sphingolipid expression in the lungs of a murine model of neutrophil elastase-induced inflammation and goblet cell metaplasia”.
Graduate Student Travel Award, to attend the American Thoracic Society International Conference (Spring 2017)
Graduate Assistantship (2017-2018)

Publications:

Sophia Karandashova, Apparao Kummarapurugu, Shuo Zheng, Bruce K. Rubin, Charles E. Chalfant, Le Kang, and Judith A Voynow. “Neutrophil elastase activity correlates with increased sphingolipid content in cystic fibrosis sputum.” In preparation for submission.

Sophia Karandashova, Apparao B. Kummarapurugu, Shuo Zheng, Charles E. Chalfant, Judith A. Voynow. “Neutrophil elastase increases airway ceramide levels via upregulation of serine palmitoyltransferase.” Submitted, under revision.

A.B. Kummarapurugu, S. Zheng, J. Ledford, S. Karandashova, J.A. Voynow. “High Mobility Group Box 1 upregulates MUC5AC and MUC5B expression in primary airway epithelial cells.” Submitted, under revision.

Florova G, Azghani AO, Karandashova S, Schaefer C, Yarovoi SV, Declerck PJ, Cines DB, Idell S, Komissarov AA. “Targeting Plasminogen Activator Inhibitor 1 in Tetracycline-Induced Pleural Injury in Rabbits.” American Journal of Physiology— Lung Cellular and Molecular Physiology. 2017 Aug 31.

Komissarov AA, Florova G, Azghani AO, Buchanan A, Boren J, Allen T, Rahman NM, Koenig K, Chamiso M, Karandashova S, Henry J, Idell S. “Dose dependency of outcomes of intrapleural fibrinolytic therapy in new rabbit empyema models.” Am J Physiol Lung Cell Mol Physiol. 2016 Aug 1;311(2):L389-99.

Marudamuthu AS, Shetty SK, Bhandary YP, Karandashova S, Thompson M, Sathish V, Florova G, Hogan TB, Pabelick CM, Prakash YS, Tsukasaki Y, Fu J, Ikebe M, Idell S, Shetty S. “Plasminogen activator inhibitor-1 suppresses profibrotic responses in fibroblasts from fibrotic lungs.” J Biol Chem. 2015 Apr 10;290(15):9428-41.

Florova G, Azghani A, Karandashova S, Schaefer C, Koenig K, Stewart-Evans K, Declerck PJ, Idell S, Komissarov AA. “Targeting of Plasminogen Activator Inhibitor 1 Improves Fibrinolytic Therapy for Tetracycline Induced Pleural Injury in Rabbits.” Am J Respir Cell Mol Biol. 2014 Aug 20.

Seidman D, Ojogun N, Walker NJ, Mastronunzio JE, Kahlon A, Hebert K, Karandashova S, Miller D, Tegels B, Marconi R, Fikrig E, Borjesson D, Carlyon JA. “Anaplasma phagocytophilum surface protein AipA mediates invasion of mammalian host cells” Cell Microbiol. 2014 Aug;16(8):1133-45.
Komissarov AA, Florova G, Azghani A, Karandashova S, Kurdowska AK, Idell S. “Active α-macroglobulin is a reservoir for urokinase after fibrinolytic therapy in rabbits with tetracycline-induced pleural injury and in human pleural fluids.” Am. J. Physiol. Lung Cell Mol. Physiol., Nov. 2013; 305(10): L682-92.

Florova G, Karandashova S, Declerck PJ, Idell S, Komissarov AA. “Remarkable Stabilization of Plasminogen Activator Inhibitor 1 in a "Molecular Sandwich" Complex.” Biochemistry. 2013 Jul 9;52(27):4697-709.

Karandashova S, Florova G, Azghani AO, Komissarov AA, Koenig K, Tucker TA, Allen TC, Stewart K, Tvinnereim A, Idell S. “Adenovirus-mediated delivery of active human PAI-1 into the pleural space exacerbates tetracycline-induced pleural injury in rabbits.” Am. J. Respir. Cell Mol. Biol., Jan. 2013; 48(1): 44-52.

Abstracts, Posters, and Presentations:

Sophia Karandashova, Apparao Kummarapurugu, Shuo Zheng, Bruce K. Rubin, Charles E. Chalfant, Le Kang, and Judith A Voynow. “Correlation between neutrophil elastase activity and concentrations of long-chain sphingolipids in cystic fibrosis sputum.” Poster, to be presented in at North American Cystic Fibrosis Conference in November 2017.

Sophia Karandashova, Apparao B. Kummarapurugu, Shuo Zheng, Bruce K. Rubin, Judith A. Voynow. “Neutrophil Elastase and Ceramide: Impact on Cystic Fibrosis Lung Inflammation.” Poster presented at the American Thoracic Society International Conference in May 2017.

A. Komissarov, A.O. Azghani, S. Karandashova, C. Schaefer, S.V. Yarovoi, P.J. Declerck, D.B. Cines, S. Idell, G. Florova. “The Slow Rate of Fibrinolyisis Limits Inhibitor Selection When Targeting Plasminogen Activator Inhibitor 1 to Enhance Intrapleural Fibrinolytic Therapy.” Am J Respir Crit Care Med. 193;2016:A2388

Galina Florova, Ali Azghani, Chris Schaefer, Serge Yarovoi, Sophia Karandashova, Douglas B. Cines, Steven Idell, Andrey A. Komissarov.“A Nearly 100-Fold Decrease in the Rate of the Reaction Between Urokinase and Plasminogen Activator Inhibitor 1 Does Not Improve the Outcome of Fibrinolytic Therapy of Tetracycline Induced Pleural Injury in Rabbits” Am J Respir Crit Care Med. 191;2015:A1136.

Andrey A. Komissarov, Galina Florova, Chris Schaefer, Sophia Karandashova, Rena Saito, Douglas B. Cines, Steven Idell . “A Peptide Derived from Plasminogen Activator Inhibitor 1 Improves the Outcome of Fibrinolytic Therapy in Tetracycline Induced Pleural Injury in Rabbits” Am J Respir Crit Care Med 191;2015:A1137.

A.A. Komissarov, G. Florova, A. Azghani, B. Smulevit, H. Kim, C. Schaefer, K. Evans, S. Karandashova, S. Huang, M. Klegerman, D.D. McPherson, S. Idell. “Imaging Ultrasound Potentiates Formation Of Intrapleural α–Macroglobulin/Urokinase Contributing To The Efficacy Of Fibrinolytic Therapy For Tetracycline-Induced Pleural Injury” Am. J. Respir. Crit. Care Med. 189; 2014:A5475.

G. Florova, A. Azghani, A. A. Komissarov, S. Karandashova, W.A. Hadden, C. Schaefer, K. Stewart-Evans, and S. Idell. “Rapid Inactivation Of Intrapleural Tissue Plasminogen Activator In Pasteurella Multocida Induced Empyema In Rabbits.” Am. J. Respir. Crit. Care Med. 187; 2013:A4311.

A. A. Komissarov, G. Florova, A. Azghani, S. Karandashova, C. Schaefer, K. Stewart, P. J. Declerck, and S. Idell. “NIH CADET 1: Elucidation of effective new strategies for fibrinolytic therapy in TCN-induced pleural injury in rabbits.” Am. J. Respir. Crit. Care Med. 187; 2013:A4312.

Andrey Komissarov, Galina Florova, Ali Azghani, Sophia Karandashova, Kris Stewart, Amy Tvinnereim, Paul Declerck, and Steven Idell. “Plasminogen Activator Inhibitor 1 Targeting Interventions In The Treatment Of Rabbits With Tetracycline-Induced Pleurodesis.” Am. J. Respir. Crit. Care Med. 185; 2012:A1571.

Andrey Komissarov, Galina Florova, Ali Azghani, Sophia Karandashova, and Seven Idell. “High Levels Of Extracellular DNA In Pleural Fluids Of Animals And Patients With Empyema Suppress Fibrinolysis.” Am. J. Respir. Crit. Care Med. 185; 2012:A1580.

Sophia Karandashova, Galina Florova, Torry Tucker, Ali Azghani, Timothy Allen, Kris Stewart, Andrey Komissarov, Amy Tvinnereim, Kathy Koenig, and Seven Idell. “Adenovirus-Mediated Delivery Of High Levels Of Plasminogen Activator Inhibitor 1 Activity To The Pleural Space Exacerbates Fibrotic Injury In Rabbits With Tetracycline-Induced Pleural Injury.” Am. J. Respir. Crit. Care Med., May 2011; 183: A2367.

Andrey Komissarov, Galina Florova, Ali Azghani, Sophia Karandashova, Kris Stewart, and Seven Idell. "Single Chain Urokinase Yields Relatively Low Grade Fibrinolytic Activity And As More Bioavailable Than Tissue Type Plasminogen Activator In Pleural Fluids Of Rabbits With Tetracycline-Induced Pleurodesis." Am. J. Respir. Crit. Care Med., May 2011; 183: A2366.

Andrey Komissarov, Galina Florova, Ali Azghani, Sophia Karandashova, Kris Stewart, Andrew Mazar, and Seven Idell. "Active Urokinase Is Protected In Vitro And In Tetracycline-induced Pleural Injury In Rabbits By An Inactive Ser195Ala Mutant." Am. J. Respir. Crit. Care Med., May 2010; 181: A4356.